NAT10 and osteosarcoma: Next, we predicted the ac4C acetylation sites in YTHDC1 mRNA with PACES (http://www.rnanut.net/paces/), as shown in Fig. 1E. The mRNA expression level of YTHDC1 was detected by qRT-PCR in U2OS and 143B osteosarcoma cell lines after NAT10 knocking down (Fig. 1F).