The trends of FBG and BW changes (Table 3) approved the T2D induction and were in line with other previous studies [25, 35, 36] .Also, we showed the serum insulin reduction in T2D rats in our previous research [35].In addition, as reported in our previous publications, food intake was increased owing to T2D (polyphagia) and we demonstrated that HIIT may modulate appetite regulation in rats with T2D through leptin signaling [35]. This evidence concerns the gene INS and type 2 diabetes mellitus.