PTK2 and glioblastoma: To recognize the potential palmitoyl acyltransferase targeting FAK, a series of siRNAs designed to target PATs were introduced into U251 cells and we found that knockdown of ZDHHC5 but not other PATs almost completely abolished the S-palmitoylation of FAK, suggesting that ZDHHC5 is the major PAT that mediates FAK palmitoylation in GBM cells (Fig. 2A).