In order to further verify the regulatory effect of miR-17-5p on JAK1, the JAK1 overexpression plasmid pcDNA3.1 JAK1 (Supplementary Figure 2) was constructed using the pcDNA3.1 (+) vector, and transfected into AML cells THP-1 and HL-60. Here, JAK1 is linked to acute myeloid leukemia.