To observe the relationship between SUCLG2-AS1 and miR-17-5p in AML cells, and to study the effect of SUCLG2-AS1 on the expression level of miR-17-5p, AML cells THP-1 and HL-60 were transfected with (a) miR-NC, (b) miR-17-5p inhibitor, (c) miR-17-5p mimics, (d) pcDNA3.1 SUCLG2-AS1 + miR-NC,and (e) pcDNA3.1 SUCLG2-AS1 + miR-17-5p mimics. This evidence concerns the gene SUCLG2 and acute myeloid leukemia.