However, we cannot exclude the possibility that some JAK2 mutations were missed due to the length of time between UKBB blood sampling and diagnosis (Supplementary Fig. 9) and the low sequencing coverage of JAK2, although these factors would equally affect the PV subgroups positive and negative for JAK2V617F in the UKBB cohort (Supplementary Fig. 3). This evidence concerns the gene JAK2 and acquired polycythemia vera.