Then the 7 candidate genes were analyzed for differential expression in HCC and normal samples (Fig. 5F), which identified CDR2L, PFKP, FSCN1 and VCAN were highly expressed in HCC samples, whereas the expressions of SPRED1, ENG and KLF2 in HCC samples showed no significant difference with that in normal samples. This evidence concerns the gene PFKP and hepatocellular carcinoma.