Finally, we identify additional SNPs with an association significance of ≤5 × 10-6 with severe ROP disease, demonstrate cross-significance with previously identified ROP-associated SNPs, and further demonstrate relevance for GLI3 and genes correlating with top SNPs within the human eye, finding expression in both human donor neurosensory retina as well as retinal pigment epithelium (RPE). The gene discussed is GLI3; the disease is retinopathy of prematurity.