Therefore, to validate the significance of GLI3 genetic variation to pre-retinal neovascular disease, we performed an extension analysis of top-associated iROP SNPs (p ≤ 5 × 10-6) to SNPs associated with DR within the Genetics of Latino Diabetic Retinopathy (GOLDR) GWAS dataset, which consists of Hispanic patients with diabetic retinopathy (DR)31,32. This evidence concerns the gene GLI3 and diabetic retinopathy.