HMGB1 and neoplasm: In vitro, LLI was shown to be taken up by MFC cells to generate ROS under laser irradiation, with the induced ROS promoting apoptosis of tumor cells and triggering ICD resulting in the release of tumor‐associated antigens and subsequent activation of immune cells.[56] The levels of ATP and HMGB1 were observed to be slightly higher in cells of LLI (+) group compared to the LI (+) group, which indicated that LLI (+) effectively elicited ICD in tumor cells.