TNF and nervous system disorder: Previous research has shown that the production of pro-inflammatory cytokines such as IL-1β, IL-6, IL-12, interferon (IFN)-γ, and TNF-α leads to the recruitment and activation of Th1 and Th17 cells on microglia, resulting in nerve fiber demyelination, which may explain the chronic muscle fatigue and neurological disorders in individuals with LCS [33, 49, 50].