showed in their study, calycosin is effective against colorectal cancer via making interacting with estrogen receptor 2 (ESR2), ATP‐binding cassette sub‐family G member 2 (ABCG2), breast cancer type 1 susceptibility protein (BRCA1), estrogen receptor 1 (ESR1), cytochrome p450 19A1 (CYP19A1), and epidermal growth factor receptor (EGFR). This evidence concerns the gene ESR1 and colorectal cancer.