Calycosin (25–200 μM) prevented the growth and viability of pancreatic cancer cells (PaCa‐2, PANC1) by triggering p21Waf1/Cip1‐induced cell cycle arrest at S phase and caspase‐dependent apoptosis via caspase‐3,8,9 activation, elevating Bax expression while lowering Bcl‐2, consequently increasing the ratio of Bcl‐2 to Bax.81 This evidence concerns the gene BAX and familial pancreatic carcinoma.