IFN‐γ's backbone folded into a more packed structure with increased ‐helix content and a higher melting temperature (Tm), which has an anti‐proliferative impact as a result of the interaction between calycosin and IFN‐γ against HepG2 cells, which are hepatocellular carcinoma, by activating caspase‐9/3.113. The gene discussed is IFNG; the disease is hepatocellular carcinoma.