Calycosin regulated MUC1, Atg5 protein expression snail protein, G1 phase arrest, and TGF‐β in MIA‐PaCa2 cell line at 50–100 μM, leading to cause apoptosis and autophagy, while at 30 mg/kg prevent tumor growth in C57/BL6 mice.82 This evidence concerns the gene ATG5 and neoplasm.