Calycosin (50 μM) therapy increased cancer cell apoptosis in the SK‐0V‐3 cell line via upregulating caspase‐3 expression and combining Cisplatin and calycosin induced apoptosis via upregulating expression of several signaling molecules such as P53, caspase‐3, caspase‐9, Bax, and Bcl‐2.99 The gene discussed is TP53; the disease is cancer.