Moreover, we conducted quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses to corroborate the discernible variations in the expression of SLC39A1 within liver cancer cells (including primary human hepatocytes L-O2, HuH7, and LM3) and SLC39A4 in pancreatic cancer cells (comprising human pancreatic duct epithelial cell line H6c7, PANC-1, and SW1990). Here, SLC39A1 is linked to liver cancer.