As shown in Fig. 2b-i, NUSAP1 was positively related to tumor mutation burden (TMB) (R = 0.3575, P < 0.0001), loss of heterozygosity (LOH) (R = 0.1954, P = 0.0139) and homologous recombination deficiency (HRD) (R = 0.4138, P < 0.0001) in PDAC. The gene discussed is NUSAP1; the disease is neoplasm.