On these premises, this study aimed to: (i) develop the serum enhanced binding (SEB) test, using all the ligands mentioned above, as a functional test of PTMs and an early biomarker of liver dysfunctions; (ii) evaluate the SEB test as well as albumin PTMs in an animal model repeatedly exposed to toxic doses of ethanol and Carbon tetrachloride (CCl4); (iii) investigate the impact of PTMs onto the structure and dynamics of albumin, (iv) interpret the results of the SEB test in light of albumin PTMs, classic hepatic laboratory tests and liver histology. This evidence concerns the gene ALB and Decreased liver function.