Many studies have confirmed the role of this pathway in other diseases and models such as Antrodan can alleviate fatty liver disease through AMPK/AAPR-γ/SIRT1 pathway56, Berberine promote adipose tissue remodeling and thermogenesis via AMPK/PPAR-γ /SIRT1 pathway57 and so on. The gene discussed is SIRT1; the disease is fatty liver disease.