EGFR and non-small cell lung carcinoma: To exclude that the observed pro-survival effects of C22orf46 were limited to the cell line NCI-H1975 or to EGFR-mutated NSCLCs, we nominated the NSCLC cell line A549, which harbors unmutated EGFR alleles (Bai et al, 2017), and generated C22orf46 knockout cell lines using the CRISPR/Cas9 technology.