EIF4EBP1 and ductal breast carcinoma in situ: Despite treatment of MCF10A cells with the mTOR activator MHY1485 [22] resulted in a small, but statistically significant, increase in the levels of phosphorylation of the mTOR downstream regulator 4EBP1 (S4A Fig), we did not observe any statistically significant change in the growth of MCF10A and MCF10A-DCIS cells on collagen I under amino acid starvation (S4B Fig), suggesting that the activation of mTORC1 is not sufficient to promote cell proliferation under starvation.