The CMV titer in the systemic leaves of N. benthamiana was higher in NbAO-silenced plants, with very low levels of CMV CP accumulation in the TRV::eGFP control and relatively no expression in the mock-treated plants (Fig. 4a), an observation that strongly suggest that NbAO_Niben101Scf03026g01009.1 gene functions as a resistant gene against CMV infection. The gene discussed is CP; the disease is cytomegalovirus infection.