The overall glycoforms were compared among three mammary carcinoma cell lines with different HA-producing abilities: parental Neu cancer cells derived from mammary tumors in mouse mammary tumor virus (MMTV)-Neu transgenic (Tg) mice and two primary breast cancer cell lines, HA-low Has2+Neo and HA-high Has2ΔNeo cells, both of which were derived from Has2 conditional Tg mice carrying the Neu transgene [22]. Here, HAS2 is linked to breast carcinoma.