Aligning with the in vitro findings, the expression of ARID5B, LINC01128 and p‐STAT3/STAT3 was upregulated, and NLRP3 inflammasome‐induced pyroptosis and apoptosis pathways were activated in mice with APS, indicating that the ARID5B‐mediated LINC01128/BTF3/STAT3 axis had an impact on accelerating APS pathogenesis in vivo. This evidence concerns the gene ARID5B and autoimmune polyendocrinopathy.