MFAP4 and Salmonella Infections: Quantification of volumes of mfap4- and tnfa-positive cells, every hour from 4 to 15 hpi, showed that Salmonella infection induced a strong tnfa response increasing over time, unlike PBS-injected controls in which only few tnfa-positive cells were observed (Figure 6A–C and Figure 6—figure supplement 1A).