To investigate the role of macrophages in the control of Salmonella infection, we ablated macrophages using Tg(mpeg1:Gal4/UAS:nfsB-mCherry) embryos and metronidazole (MTZ) treatment (Nguyen-Chi et al., 2020; Figure 3—figure supplement 2A, B). Here, MPEG1 is linked to Salmonella Infections.