METTL3 and laryngotracheoesophageal cleft: To continue to systematically investigate the potential role of METTL3 in LC, we successfully constructed knockdown and overexpression of METTL3 at the cellular level by transfecting two human LC cell lines, A549 and PC9, with two independent siRNAs (named si-METTL3 1# and si-METTL3 2#) and METTL3 overexpression plasmids (named oe-METTL3), respectively (Figure 2a and b).