To confirm that neddylation pathway regulated the homeostasis of DN T cells via Bim, we generated the double KO lupus-prone mice (Ube2m-/-Bim-/-lpr) and found that the deficiency of Bim disrupted the apoptosis of DN T cells for Ube2m deficiency and cannot inhibit the development of lupus. The gene discussed is UBE2M; the disease is systemic lupus erythematosus.