Further exploration of ALKBH5's protein distribution, interactors, and expression in GBMs reveals its presence in both the nucleus and cytoplasm of U251 GBM cells (Fig. 4A) Immunohistochemistry staining images from the HPA datasets suggest higher expression of ALKBH5 in higher-grade gliomas (Fig. 4B). This evidence concerns the gene ALKBH5 and central nervous system cancer.