Di et al. (2018) reported that by directly targeting Dickkopf-related protein 1, the negative feedback inhibitor in Wnt/β-actin signaling, miR-146a knockout could reverse the excess osteogenic potential in ankylosing spondylitis fibroblasts.13 However, the speculated upregulation of RUNX2 via the Wnt/β-actin pathway after miR-146a overexpression was not observed in hDPSCs, which needs further investigation. Here, ACTB is linked to ankylosing spondylitis.