Subsequently, we detected the expression of T cell-related markers (tcrα, tcrβ, cd8α, d8β, cd4-1,), B cell-related markers (igd, igm, igt), myeloid cell-related markers (ncf2, cd11b, mpeg1, and mpo), chemokines (ccr10, ccl20b, ccl9, cxcl13, and ccl25), as well as interleukins (il1β, il8, il10b, and il6) in the OM and found the expression of these immune-related genes also decreased significantly in the antibiotics treatment group (Fig. 4d). This evidence concerns the gene CCL25 and ocular melanoma.