Against the background that HA improves ocular tissue distribution and inhibition of neovascularization by ANP potentially reduces tumor growth, the goal of the study presented was to evaluate the applicability of ANP‐conjugated, HA‐coated gold nanoparticles (ANP‐HA‐GNPs) as promising candidate nanocarriers for the treatment of chemoresistant RB tumors in vivo. This evidence concerns the gene NPPA and neoplasm.