AKT1 and Wilson disease: To examine whether the uncovered proteomic alterations give rise to changes in the dynamics of HGF-induced signal transduction in WD primary hepatocytes, we designed based on our previous knowledge of the cross-talk of MAPK- and PI3K/AKT-pathways (D’Alessandro et al, 2015) and the link to proliferation (Mueller et al, 2015), dose- and time-resolved experiments to delineate by quantitative immunoblotting the differences in HGF-induced responses in WD and SD primary hepatocytes (Fig. 2A).