CD8-Tem1 that had higher expression of GZMB and CTSW were enriched in bone metastatic tumors, whereas CD8-Tem2 cells were enriched in primary ccRCC with higher expression of KLRD1, KLRF1, and KLRG1 (Fig. 4d and Supplementary Fig. 5c). This evidence concerns the gene CD8A and nonpapillary renal cell carcinoma.