CRISPRi suppression of NF2 in NF1-mutant NF95.11b neurofibroma cells induced PAK1 phosphorylation (pPAK1) without significantly affecting pMEK, pERK, or pAKT compared to sgNTC (Fig. 3c and Supplementary Fig. 9c). This evidence concerns the gene PAK1 and plexiform neurofibroma.