CRISPRi suppression of NF2 in NF1-mutant NF95.11b neurofibroma cells induced PAK1 phosphorylation (pPAK1) without significantly affecting pMEK, pERK, or pAKT compared to sgNTC (Fig. 3c and Supplementary Fig. 9c). Here, NF2 is linked to neurofibroma.