sgRNAs targeting tumor suppressor genes such as TP53 or NF2 that were lost in Schwann cell tumors (Fig. 1a) were significantly enriched in both selumetinib and vehicle control conditions, suggesting tumor suppressor loss promotes cell growth and may also mediate selumetinib responses in NF1-mutant, PRC2-intact neurofibroma cells. This evidence concerns the gene TP53 and neurofibroma.