Moreover, FBXO31 protein expressions were detected by Western blotting between human normal HPDE6-C7 pancreatic epithelial cell line and human PC cell lines, including Panc-1, PaTu-8988, SW1990, CFPAC-1, BxPC-3 and Capan-1 cells. This evidence concerns the gene FBXO31 and pachyonychia congenita.