In our study, for the first time, the effects of the LDH-A inhibitor+docetaxel combination were investigated in prostate cancer murine model, and it was found that adding the LDH-A inhibitor to Docetaxel had a positive effect on both protein expression and mRNA levels, statistically significantly decreasing the Snail, Slug, and Twist transcription factors. This evidence concerns the gene TWIST1 and prostate carcinoma.