Using the proliferation of the BT474 breast cancer cell line as a read-out, it was shown that CPT1A-mediated succinylation inhibited the catalytic activity of ENO1, but promoted glutamine-independent cell proliferation, a rescue mechanism of ENO-deficient MDA-MB231 breast cancer cells that had previously been reported [28, 97]. The gene discussed is CPT1A; the disease is breast cancer.