We previously designed adenovirus AdV-D24-inducible costimulator ligand (ICOSL)-CD40L (AdV1), which selectively replicated in cancer cells but not in healthy cells and was armed with two potent costimulatory molecules: inducible costimulator ligand (ICOSL) and CD40 ligand (CD40L, CD154) (Garofalo et al., 2021). This evidence concerns the gene CD40LG and cancer.