Nf1 and Trp53 alterations were present in both models, but neither model exhibited Idh1/2, Atrx, Braf, H3f3a mutations nor copy number alterations of Pten, Egfr, Nf1, or Rb1, all of which are commonly associated with huGBM and astrocytoma. This evidence concerns the gene TP53 and astrocytoma (excluding glioblastoma).