In differentiated KD3 myotubes, cystogenic strains of T. gondii can be matured over a period of four weeks, during which the cysts develop structural and functional traits of in vivo cysts (Christiansen et al., 2022) and express bradyzoite and cyst markers, such as BAG1, CC2, glycans, a discernible cyst wall, as well as amylopectin granules. This evidence concerns the gene BAG1 and cyst.