By contrast, defining mutations of AML-MR in WHO5th were overall rare (range 0.1–2.1%), frequently co-occurred with other defining alterations (for example, EZH2 in PICALM::MLLT10), and could be found in various clusters rather than as a distinct group (Extended Data Fig. 3a,f), leading to its exclusion as a defining category for pAML. The gene discussed is EZH2; the disease is acute myeloid leukemia.