Five of the subunits (TCP1, CCT3, CCT4, CCT6A and CCT7) are putative GBM uEV diagnostic proteins with significantly higher levels in GBM Pre-OP uEVs compared to HC (FC ≥ 2, p-val≤0.05; Fig. 5b; Supplementary Table 5) and were previously reported in GBM-EVs derived in vitro cells, from neurosurgical aspirates (CUSA) and plasma (Table 2). This evidence concerns the gene TCP1 and glioblastoma.