Next, the TRiC protein subunits and 33 interacting proteins were annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Reactome Pathways by The Database for Annotation, Visualisation and Integrated Discovery (DAVID) and revealed significant enrichments in ‘pathways in cancer’ (4.5-fold, p-val = 0.0028), ‘PI3K/Akt signaling pathway’ (7.7-fold, p-val = 0.000035), ‘signaling by WNT’ (4.2-fold, p-val = 0.027), ‘Beta-catenin independent WNT signaling’ (9.6-fold, p-val = 0.016) and ‘signaling by Hedgehog’ (9.4-fold, p-val = 0.017; Fig. 5c). This evidence concerns the gene AKT1 and cancer.