There was no difference in the density of T regulatory cells, M1/M2 macrophages (CD68+, CD163+, CD206+), CD11+, TCF1+, and SMA+ cells (Data Supplement, Fig S16), as well as in PD-L1 expression on tumor cells and nontumor cells, and in total between pre- and post-ICI samples as assessed by mIF (Data Supplement, Fig S17), or in PD-L1 expression as assessed by IHC (Data Supplement, Fig S18). This evidence concerns the gene CD163 and neoplasm.