TRIM56 and metabolic dysfunction-associated steatotic liver disease: We observed that the protein expression level, rather than the gene expression level, of TRIM56 was markedly downregulated in mice fed a high-fat diet (HFD) as well as in individuals with NAFLD, as revealed by Western blot analysis and immunofluorescence staining (Figure 1, L–O, and Supplemental Figure 2, A–C).