We further evaluated the expression level in LIHC using LIHCDB (Integrative Molecular Data base of Hepatocellular Carcinoma, http://lifeome.net/datebase/hccdb/home.html) and found that TM4SF1, TM4SF4, TM4SF5, TM4SF18, and TM4SF19 were all significantly more highly expressed in LIHC from most cases of GEO and TCGA data than in normal adjacent tissues (Figure 2G–2L). This evidence concerns the gene TM4SF5 and hepatocellular carcinoma.