We identified the effect of transfection with antagomiR‐320a on the tumor growth of transplanted SiHa cells with knockdown HPV16 E6, and found that knockdown of HPV16 E6 decreased the tumor volume and weight of transplanted SiHa cells, down‐regulated Ki‐67 expression, and increased the apoptosis rate of transplanted tumor cells. Here, MKI67 is linked to neoplasm.