The study also tested the nano-PTA in spheroids of non-tumorous cell lines, and besides proving that the internalization of the nanomedicine was less effective compared to tumor spheroids, it was also observed that under the same treatment conditions, non-tumorous spheroids exhibited less pronounced effects, assuming the efficiency of HGNs@anti-MUC1 to specifically treat tumors. Here, MUC1 is linked to neoplasm.