To evaluate the effect of leptin-PEG-FBP on Fas-mediated apoptosis, hypoxic Neuro2a mouse neuroblastoma (N2a) cells were treated with FasL (0.1 mM), followed by a treatment with the peptides indicated in Figure 1B. Our results showed that both FBP and leptin-PEG-FBP significantly reduced cell death upon exposure to FasL, as indicated by the 40% decrease in Annexin V positivity (Figure 1B). Here, ANXA5 is linked to neuroblastoma.