For example, AAV-Klotho was injected into the bilateral hippocampus of rats with a model of temporal lobe epilepsy, and after 9 weeks, it was found that AAV-Klotho induced Klotho overexpression in the hippocampus, effectively improved cognitive impairment, and had a neuroprotective effect. The gene discussed is KL; the disease is temporal lobe epilepsy.