To elucidate how RCJ reduced colitis severity, the expression of pro- and anti-inflammatory cytokines and chemokines was quantified using a cytokine array (IL-1α, IL-1β, IL-3, IL-6, IL-10, IL-16, IL-17, IL-23, IL-27, CCL1, CXCL1, CXCL9, CXCL10, CXCL11, G-CSF, GM-CSF, TNF-α, and IFN-γ). This evidence concerns the gene TNF and colitis.