Matrine had a time-dependent proliferation inhibitory effect on CHMm and CHMp cells within a safe concentration range and CETSA findings indicated that matrine could increase the heat stability of BTF3 protein; biotin-labeled matrine probe with CHMm and CHMp cells at safe concentrations had a substantial impact, indicated that BTF3 was abundantly expressed in canine mammary tumor cells. The gene discussed is BTF3; the disease is breast cancer.