In addition, the analyses by Tumor Immune Estimation Resource (TIMER) software showed that the GLIS1 mRNA expression exhibited positive correlations with the infiltration of B cells (R = 0.164, p < 0.001), CD8+ T cells (R = 0.214, p < 0.001), CD4+ T cells (R = 0.335, p < 0.001), macrophages (R = 0.469, p < 0.001), neutrophils (R = 0.235, p < 0.01), and DC (R = 0.297, p < 0.001), but had a negative correlation with the purity of tumor (R = −0.499, p < 0.001) (Figure 6C). This evidence concerns the gene CD8A and neoplasm.