In this study, we conducted comprehensive research on the potential mechanism of luteolin’s (Lut) regulation of TRPV1 and CYP2A13 in the treatment of COPD, using cigarette smoke extract (CSE)-treated A549 cells and cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced COPD mouse models. Here, TRPV1 is linked to chronic obstructive pulmonary disease.