RANBP2 and myeloid leukemia: To determine whether RanBP2 regulates ISG expression in immune cell lines, we depleted RanBP2 in human myeloid leukemia mononuclear (THP-1) cells using lentiviral delivered shRNA (“RanBP2 shRNA3”, Figure 2F) and examined the expression of ISG mRNAs induced by IFN-α.