Immunoblot analysis confirmed these findings, showing significant reductions in ZO-1 and occludin expression compared to the DNBS challenge, both in the acute phase (−70% **** p < 0.0001 and −80% **** p < 0.0001 vs. respective vehicle groups for ZO-1) and resolution phase of colitis (−75% **** p < 0.0001 and −70% **** p < 0.0001 vs. respective vehicle groups for occludin). Here, OCLN is linked to colitis.